Member Profile
Membership type: full
Ery Odette Fukushima
Country of origin: Bolivia Currently in: Ecuador, Tena, Napo General field of specialization: Structural, Cell and Molecular Biology-
Degrees
2013 Doctorate Structural, Cell and Molecular Biology -
Current Research Activities
Structural, Cell and Molecular Biology
I am working on plant specialized metabolites with a particular focus on how these compounds are produced in plants (the enzymes responsible for their biosynthesis), their possible roles in plants (why plants produce them), how different they are from each other and how diverse they could be (their evolutionary relationships). I believe the elemental findings on these topics could be applied in agriculture and pharmaceutical industries. For all of this research, I am using techniques related to metabolomics, transcriptomics, molecular biology, plant tissue culture as well as genome editing techniques. Recently, I have also been studying the potential biological activities of these compounds and the structural features involved in these activities.
Publications resulting from Research:
Peer-reviewed original research articles
1. Srisawat P., Yasumoto S., Fukushima E.O., Robertlee J., Seki H., Muranaka T. (2020) Production of the bioactive plant‐derived triterpenoid morolic acid in engineered Saccharomyces cerevisiae. Biotechnol. Bioeng. https://doi.org/10.1002/bit.27357 (impact factor 4.26)
2. Vo N.N.Q., Nomura Y., Muranaka T., Fukushima E.O. (2019) Structure-activity relationships of pentacyclic triterpenoids as inhibitors of cyclooxygenase and lipoxygenase enzymes. J. Nat. Prod. doi: 10.1021/acs.jnatprod.9b00538. (impact factor 1.67)
3. Fanani M.Z., Fukushima E.O., Sawai S., Tang J., Ishimori M., Sudo H., (2019) Molecular basis of C-30 product regioselectivity of legume oxidases involved in high-value triterpenoid biosynthesis. Front. Plant Sci.10:1520. (impact factor 4.016)
4. Suzuki H., Fukushima E.O., Shimizu Y., Seki H., Fujisawa Y., Ishimoto M., Osakabe K., Osakabe Y., Muranaka T. (2019) Lotus japonicus triterpenoid profile and characterization of the CYP716A51 and LjCYP93E1 genes involved in their biosynthesis in planta. Plant Cell Physiol. 60(11): 2496-2509 (impact factor 4.59)
5. Srisawat P., Fukushima E.O., Yasumoto S., Robertlee J., Suzuki H., Seki H., Muranaka T. (2019) Identification of oxidosqualene cyclases from the medicinal legume tree Bauhinia forficata: a step toward discovering preponderant α-amyrin-producing activity. New Phytol. ;224(1):352-366 (impact factor 7.29)
6. Religia P., Kato Y., Fukushima E.O., Matsuura T., Muranaka T., Watanabe H. (2019) Atrazine exposed phytoplankton causes the production of non-viable offspring on Daphnia magna. Mar. Environ. Res. 145:177-183 (impact factor 3.815)
7. Suzuki H.*, Fukushima E.O.*, Seki H., Muranaka T. (2018) Comparative analysis of CYP716A subfamily enzymes for the heterologous production of C-28 oxidized triterpenoids in transgenic yeast. Plant Biotechnol. 35:131-139 (*equal contribution, impact factor 1.6)
8. Muangphrom P., Seki H., Matsumoto S., Nishiwaki M., Fukushima E.O., Muranaka T. (2018) Identification and characterization of a novel sesquiterpene synthase, 4-amorphen-11-ol synthase, from Artemisia maritima. Plant Biotechnol. 35:113-121 (impact factor 1.6)
9. Yasumoto S., Seki H., Shimizu Y., Fukushima E.O. and Muranaka T. (2017) Functional Characterization of CYP716 Family P450 Enzymes in Triterpenoid Biosynthesis in Tomato. Front. Plant Sci. 8:21. doi: 10.3389/fpls.2017.00021 (3 citations, impact factor 4.495)
10. Vo N.N.Q., Fukushima E.O., Muranaka T. (2017) Structure and hemolytic activity relationships of triterpenoid saponins and sapogenins. J. Nat. Med. 71:50-58 (6 citations, impact factor 1.67)
11. Muangphrom P., Seki H., Suzuki M., Komori A., Nishiwaki M., Mikawa R., Fukushima E.O., Muranaka T. (2016) Functional analysis of amorpha-4,11-diene synthase (ADS) homologs from non-artemisinin-producing Artemisia species : the discovery of novel koidzumiol and (+)-α-bisabolol synthases. Plant Cell Physiol. 57 (8):1678-1688 (1 citation, impact factor 4.319)
12. Muangphrom P., Seki H., Fukushima E.O., Muranaka T. (2016) Artemisinin-based antimalarial research: application of biotechnology to the production of artemisinin, its mode of action, and the mechanism of resistance of Plasmodium parasites. J. Nat. Med. 70(3):318-334 (15 citations, impact factor 1.67)
13. Yasumoto S., Fukushima E.O., H. Seki, and T. Muranaka (2016) Novel triterpene oxidizing activity of Arabidopsis thaliana CYP716A subfamily enzymes. FEBS lett. 590(4): 533-540. (14 citations, impact factor 3.519)
14. Khakimov B., Kuzina-Poulsen V., Erthmann P. Ø., Fukushima E. O., Augustin J.M., Olsen C.E., Scholtalbers J., Volpin H., Andersen S. B., Hauser T. P., Muranaka T. and Bak S. (2015) Identification and genome organization of saponin pathway genes from a wild crucifer and their use for transient production of saponins in tobacco. Plant Journal 84(3): 478-490. (24 citations, impact factor 5.468)
15. Muangphrom P., Suzuki M., Seki H., Fukushima E. O. and Muranaka T. (2014). Functional analysis of orthologous artemisinic aldehyde Δ11(13)-reductase reveals potential artemisinin-producing activity in non-artemisinin-producing Artemisia absinthium. Plant Biotechnology 31: 483-491. (4 citations, impact factor: 0.935)
16. Fukushima E.O., Seki H., Sawai S., Suzuki M., Ohyama K., Saito K. and Muranaka T. (2013) Combinatorial biosynthesis of legume natural and rare triterpenoids in engineered yeast. Plant Cell Physiol. 54: 740-749. (60 citations, impact factor: 4.319)
17. Kunii M., Kitahama Y., Fukushima E. O., Seki H., Muranaka T., Yoshida Y., and Aoyama Y. (2012) β-Amyrin oxidation by oat CYP51H10 expressed heterologously in yeast cells: The first example of CYP51-dependent metabolism other than the 14-demethylation of sterol precursors. Biol. Pharm. Bull. 35: 801-804. (19 citations, impact factor: 1.26)
18. Seki H., Sawai S., Ohyama K., Mizutani M., Ohnishi T., Sudo H., Fukushima E.O., Aoki T., Saito K., Muranaka T. (2011) Triterpene functional genomics in licorice for identification of CYP72A154 involved in the biosynthesis of glycyrrhizin. Plant Cell 11: 4112-4123. (137 citations, impact factor: 10.529)
19. Fukushima E.O., Seki H., Ohyama K., Ono E., Umemoto N., Mizutani M., Saito K., Muranaka T. (2011) CYP716A subfamily members are multifunctional oxidases in triterpenoid biosynthesis. Plant Cell Physiol. 52: 2050-2061. (138 citations, impact factor: 4.319)
20. Almanza G.R., R. Quispe, P. Mollinedo, G. Rodrigo, E.O. Fukushima, R. Villagomez, B. Akesson, O. Sterner (2011) Antioxidant and antimutagenic polyisoprenylated benzophenones and xanthones from Rheedia acuminata. Nat. Prod. Com. 6: 1269-74. (7 citations)
21. 福島エリオデット、關 光、村中俊哉: 植物テルペノイド代謝の多様性とコンビナトリアル生合成に向けて、生物工学会誌、Vol. 91: 337-341 (2013)
Book chapters
1. Fukushima E.O., Seki H and Muranaka T. (2014) Heterologous expression of triterpene biosynthetic genes in yeast and subsequent metabolite identification through GC-MS In: Plant Isoprenoids. Manuel Rodríguez (Ed.) Methods Mol. Biol. 1153: 235-43.
2. Fukushima E.O, Seki H and Muranaka T. (2014) Plant cytochrome P450s in triterpenoid biosynthesis: Diversity and application to combinatorial biosynthesis in: fifty years of Cytochrome P450 research. Hiroshi Yamazaki (Ed.). Springer Japan. pp.125-133. (2 citations)
Current profession
Current professional activities type:ResearchTeachingI am currently working as Associate Professor in the Universidad Regional Amazónica Ikiam in the Ecuadorian Amazonia. I work on plant metabolites occurring and plant biotechnology approaches that may help the development of the region.
Affiliations
Osaka UniversityPresentation given
Event: -
Other Awards
Aug 2012JSPCMB encouragement award for students, from the Japanese Society for Plant Cell and Molecular BiologyJSPCMB encouragement award for students, from the Japanese Society for Plant Cell and Molecular Biology by the research entitled “Research of triterpenoid biosynthetic enzymes in higher plants”Aug 2016Falling Walls Lab TokyoThird place at Falling Walls Lab Tokyo. Science communication contest organized by the German Research & Innovation Forum Tokyo and EURAXESS Links Japan and supported by the Falling Walls Foundation, A.T. Kearney and Festo.
