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Member Profile

Membership type: full

Luisa Marcela Villamil Diaz

Country of origin: Colombia Currently in: Colombia, Cajicá General field of specialization: Biological Systems and Organisms
Academic Background


2003 Doctorate Biological Systems and Organisms
Research and Profession

Current Research Activities

Biological Systems and Organisms

I work in disease prevention, nutrition, and analysis of immunological responses under different stressors and treatments. I am interested in the mass production of new-generation probiotics and symbiotics as prophylactics for disease control and reducing antibiotic use, which adversely affects the aquatic environment and consumers. I am also working in biotechnology studies to evaluate the bioactive potential of microorganisms in different applications such as sunscreen formulations, new antibiotic molecules, and other activities of interest to the industry.

Publications resulting from Research: 

• Melo-Bolívar JF., Pardo RY, Quintanilla-Carvajal M.X, Díaz LE, Hume ME, Villamil, L. 2023. Development of a Nile tilapia (Oreochromis niloticus) gut microbiota-derived bacterial consortium with antibacterial activity against fish pathogens. Innovative Food Science and Emerging Technologies, 83, 103214.
• Sánchez-Suárez J, Díaz L, Coy-Barrera E, & Villamil L. 2022. Specialized Metabolism of Gordonia Genus: An Integrated Survey on Chemodiversity Combined with a Comparative Genomics-Based Analysis. BioTech,11(4), 53. MDPI AG. Retrieved from
• Villegas-Plazas M, Villamil L, Martínez-Silva MA, González-Jiménez T, Salazar M, Güiza L, Junca H. 2022. Microbiome composition and autochthonous probiotics from contrasting probiosis/dysbiosis states in cobia (Rachycentron canadum) fish epitheliocystis. Access Microbiology, 4(8), 000405.
• La Hoz-Romo C, Díaz L, Villamil L. 2022. Marine Actinobacteria a New Source of Antibacterial Metabolites to Treat Acne Vulgaris Disease—A Systematic Literature Review. Antibiotics,11(7), 965.
• Melo-Bolívar J, Ruiz Pardo R, Junca H, Sidjabat H, Cano-Lozano J, &Villamil L. 2022. Competitive Exclusion Bacterial Culture Derived from the Gut Microbiome of Nile Tilapia (Oreochromis niloticus) as a Resource to Efficiently Recover Probiotic Strains: Taxonomic, Genomic, and Functional Proof of Concept. Microorganisms, 10(7), 1376.
• Sánchez-Suárez J, Díaz L, Junca H, Garcia-Bonilla E, Villamil L. 2022. Microbiome composition of the marine sponge Cliona varians at the Neotropical Southern Caribbean Sea displays a predominant core of Rhizobiales and Nitrosopumilaceae. J Appl Microbiol. 2022 Jul 12. Doi: 10.1111/jam.15714. PMID: 35818766.
• Sánchez-Suárez J, Díaz L, Melo-Bolivar J, Villamil L. 2022. Draft genome sequence data of Gordonia hongkongensis strain EUFUS-Z928 isolated from the octocoral Eunicea fusca. Data in Brief, 42, 108076.
• Sánchez-Suárez J, Villamil L, Díaz L, & Coy-Barrera E. 2022. Uncovering Streptomyces-Derived Compounds as Cosmeceuticals for the Development of Improved Skin Photoprotection Products: An In Silico Approach to Explore Multi-Targeted Agents. Scientia Pharmaceutica, 90(3), 48.
• Cano-Lozano J, Villamil L, Bolivar J, Hume M, Pardo R. 2021. Probiotics in tilapia (Oreochromis niloticus) culture: Potential probiotic Lactococcus lactis culture conditions. Journal of bioscience and bioengineering.
• Sánchez-Suárez J, Garnica-Agudelo M, Villamil L, Díaz L, Coy-Barrera E. 2021. Bioactivity and Biotechnological Overview of Naturally Occurring Compounds from the Dinoflagellate Family Symbiodiniaceae: A Systematic Review. ScientificWorldJournal. 2021 Dec 17;2021:1983589. doi: 10.1155/2021/1983589. PMID: 34955690; PMCID: PMC8709762.
• Sánchez-Suárez J, Villamil L, Coy-Barrera E, Díaz L. 2021. Cliona varians-Derived Actinomycetes as Bioresources of Photoprotection-Related Bioactive End-Products. Mar Drugs. 2021 Nov 27;19(12):674. doi: 10.3390/md19120674. PMID: 34940673.
• Moncaleano-Niño, A. M., Gómez-Cubillos, M. C., Luna-Acosta, A., Villamil, L., Casseres-Ruiz, S., Ahrens, M. J. 2022. Monitoring metallothionein-like protein concentrations and cholinesterase activity in tropical cup oysters as biomarkers of exposure to metals and pesticides in the southern Caribbean, Colombia. Environmental Science and Pollution Research 29 (17), 25157-25183.
• Melo-Bolívar J, Ruiz R, Hume M, Villamil L. 2021 Multistrain probiotics use in main commercially cultured freshwater fish: A systematic review of the evidence. Reviews in Aquaculture. DOI: 10.1111/raq.12543.
• Sánchez-Suarez J, Coy-Barrera E, Villamil L, Díaz L. 2020. Streptomyces-derived metabolites with potential photoprotective properties: A Systematic Literature Review and Meta-Analysis. Molecules. 25(14), 3221.
• Toquica J, Becerra-Real L, Villamil L. 2020. Effect of Bacillus firmus C101 on the growth of Litopenaeus vannamei Boone (White Shrimp) post-larvae, and Brachionus plicatilis s.s. Müller (Rotifer). Boletín de Investigaciones Marinas y Costeras 49 (1), 63-80 DOI: 1025268/bimc.invemar.2020.49.1.774.
• Melo-Bolívar J, Ruiz-Pardo R, Hume M, Sidjabat H, Villamil L. 2020. Probiotics for cultured freshwater fish. Microbiology Australia 41(2) 105-108
• Melo-Bolívar J, Ruiz-Pardo R, Hume M, Nisbet D, Rodríguez-Villamizar F, Alzate J, Junca H, Villamil L. 2019. Establishment and characterization of a competitive exclusion bacterial culture derived from Nile tilapia (Oreochromis niloticus) gut microbiomes showing antibacterial activity against pathogenic Streptococcus agalactiae. PLoS ONE 14(5): e0215375.
• Villamil L, Infante Villamil S, Rozo G. 2019. Effect of dietary administration of kappa carrageenan extracted from Hypnea musciformis on innate immune response, growth, and survival of Nile tilapia (Oreochromis niloticus) Aquaculture International 27: 53.
• Moncaleano-Niño A, Luna-Acosta A, Gómez-Cubillos M, Villamil L⁠, Ahrens M. 2018. Cholinesterase activity in the cup oyster Saccostrea sp. exposed to chlorpyrifos, imidacloprid, cadmium and copper. Ecotoxicology and Environmental Safety 151: 242-254. 10.1016/j.ecoenv.2017.12.057
• Aguirre-Rubí J, Luna-Acosta A, Ortiz-Zarragoitia M, Zaldibar B, Izagirre U, Ahrens M, Villamil L, Marigómez, I. 2017. Assessment of ecosystem health disturbance in mangrove-lined Caribbean coastal systems using the oyster Crassostrea rhizophorae as sentinel species. The Science of the total environment. doi: 10.1016/j.scitotenv.2017.08.098.
• Moncaleano-Niño A, Barrios-Latorre J, Poloche-Hernández V, Becquet H, Villamil L, Thomas-Guyon, Ahrens M, Luna-Acosta M. 2017. Alterations of tissue metallothionein and vitellogenin concentrations in tropical cup oysters (Saccostrea sp.) following short-term (96 h) exposure to cadmium. Aquatic Toxicology 185:160-170. DOI: 10.1016/j.aquatox.2017.02.011
• Villamil L, Esguerra D. 2017. Enterococcus, Myroides y Exiguobacterium: Géneros bacterianos con potencial probiótico para el cultivo de tilapia nilotica (Oreochromis niloticus). Acta Biológica Colombiana 22(3):331-339.
• Nikapitiya C, McDowell I, Villamil L, Muñoz P, Sohn S, Gómez-Chiarri M. 2014. Identification of potential general markers of disease resistance in American oysters, Crassostrea virginica through gene expression studies. Fish and Shelfish Immunology 41, (1) 27-36. S1050-4648(4)00214-9. doi: 10.1016/j.fsi.2014.06.015
• Villamil L, Mejía L, Martínez-Silva M, Díaz J, Suarez S, Salazar M, Güiza L , Mendoza M. 2012. El pez que promete salvar la acuicultura marina en Colombia. Expeditio 7- 17 ISSN:2145-6836.
• Villamil L, Martínez-Silva M, Reyes C. 2012. In vivo and in vitro assessment of Lactobacillus acidophilus as probiotic of tilapia culture improvement. Aquaculture Research DOI: 10.1111/are.12051
• Villamil L, Infante S, Lecompte O. 2011.Uso de microorganismos benéficos en el alimento vivo para controlar la aparición de enfermedades durante el cultivo de animales acuáticos. MUTIS 1 96-113.
• Murillo I, Villamil L. 2011. Bacillus cereus and Bacillus subtilis use as probiotics in rotifer (Brachionus plicatilis) culture. Journal of Aquaculture Research and Development, Special Issue "Prebiotic & probiotic applications in aquaculture".1-5. ISSN:2155-9546 . doi:10.4172/2155-9546.S1-007
• Villamil L, Planas M, Novoa B. 2011. Pediococcus acidilactici in the culture of turbot larvae: administration pathways. Aquaculture 307: 83-88. DOI information: 10.1016/j.aquaculture.2010.07.004
• Dorrington T, Villamil L, Gomez-Chiarri M. 2011. Antimicrobial activity and up regulation in response to infection of oyster histone H4. Fish and Shellfish Immunology. 30(1):94-101 DOI 10.1016/j.fsi.2010.09.006
• Villamil L, Sanjuan A, Martinez Silva MA, Ospina A, Reyes C. 2010. Evaluación de bacterias probióticas sobre la expresión de genes de defensa, resistencia a infecciones e incremento en peso de tilapia nilótica, Oreochromis niloticus. Memorias de Investigación 2009. Tercer Seminario de Investigación. Universidad Jorge Tadeo Lozano, p 56-58. ISSN2027-2065.
• Villamil L, Martínez-Silva M. 2009. Probióticos como una herramienta biotecnológica en el cultivo de camarón: Reseña. Bol. Invest. Mar. Cost. 38 (2) 151-169.
• Gómez-León J, Villamill L, Salger SA, Sallum RH, Remacha-Triviño A, Leavitt DF, Gómez-Chiarri M. Survival of eastern oysters Crassostrea virginica from three lines following experimental challenge with bacterial pathogens. Dis Aquat Organ. 2008 Apr 1;79(2):95-105. doi: 10.3354/dao01902. PMID: 18500026.
• Remacha-Trivino A, Gómez- León J, Villamil L, Borsay-Horowitz D, Dungan, C, Gual-Arnau X, Gómez-Chiarri M. 2008. Numerical quantification of trophozoites of Perkinsus marinus (Perkinsozoa) in the Eastern Oyster Crassostrea virginica (Mollusca: Bivalvia) by the application of modern stereological techniques. I. Natural populations. J. Parasitology 94 (1), 125-136. ISSN: 0022-3395.
• Villamil L, Gomez-Leon J, Gomez-Chiarri M. 2007. Role of nitric oxide in the defenses of Crassostrea virginica to experimental infection with the protozoan parasite Perkinsus marinus. Developmental and Comparative Immunology. 31(10) 968-977. ISSN: 0145-305X
• Díaz-Pulido G, Villamil L. 2007. Herbivory effects on the morphology of the brown alga Padina boergesenii (Phaeophyta). Journal of Phycology. 46 (2):131-136. (Print ISSN: 0031-8884 ). DOI: 10.2216/05-60.1
• Gómez-León J, Villamil L, Lemos L, Novoa B, Figueras A. 2005. Isolation of Vibrio alginolyticus and Vibrio splendidus associated to mass mortalities of clams (Ruditapes decussatus) in a commercial hatchery. Applied and Environmental Microbiology. 71:98-104. doi: 10.1128/AEM.71.1.98-104.2005
• Villamil L, Figueras A, Aranguren R , Novoa B. 2003. Non-specific immune responses of turbot (Scopthalmus maximus L.) experimentally infected with a pathogenic Vibrio pelagius like. Journal of Fish Diseases. 26: 321-329.
• Villamil L, Figueras A, Toranzo A, Planas M, Novoa B. 2003. Isolation of a highly pathogenic Vibrio pelagius like strain associated to mass mortalities of turbot Scophthalmus maximus) (L), larvae. Journal of Fish Diseases 26: 293-303.
• Villamil L, Figueras A, Planas M, Novoa B. 2003. Control of Vibrio alginolyticus in Artemia culture by treatment with bacterial probiotics. Aquaculture 219: 43-56.
• Villamil L, Figueras A, Novoa B. 2003. Immunomodulatory effects of nisin in turbot (Scophthalmus maximus). Fish and Shellfish Immunology 14: 157-169.
• Villamil L, Tafalla C, Figueras A, Novoa B. 2002. Evaluation of immunomodulatory effects of some lactic acid bacteria in turbot (Scophthalmus maximus). Clinical and Diagnostic Laboratory Immunology Vol 9, N.6: 1318-1323.

Current profession

Current professional activities type: 
I am currently the director of the Ph.D. program in Biosciences at Universidad de La Sabana, teach and do research in biotechnology, bioprospecting and aquaculture

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