Amira Hesham Ayad Dr. Amira Ayad
About me
Many plants around the world suffer significantly from plant parasitic nematodes, so transgenic crops were suggested as an application of biotechnology to make plants more resistant to nematodes in a safe way and without causing any changes in the ecosystem of plants. There are a few strategies for controlling nematodes in cucumbers, and one of these methods uses natural resistance genes (R-genes) to resist nematodes. Our study was conducted on an isolated mi gene from nematode-resistant tomato plants, which produce proteins capable of preventing nematode feeding, and was detected with the PCR method. Then, the resistant gene was inserted into the pPT7 vector and carried out in chitosan nanoparticles to be transformed into plants (cucumber cultivars) using injections. The transgenic plants were tested by using a spore suspension on a leaf. As a result of this study, a UV/visible spectroscope was used to characterize chitosan nanoparticles. The GFP gene fluorescent light is found in transgenic plants compared to the control. This will be a new approach to controlling nematodes, not only with genetic engineering but also with chitosan nanoparticles to get new cucumber cultivars to resist nematodes.
In the current study, a new transformation method was used to transform the mi gene (resistant nematode gene) using chitosan particles. The aim was to transfer the nematode-resistant genes from nematode-resistant plants such as cultivars (Solanum lycopersicum, Type D, Hybrid Ajyad 7, Hybrid Ajyad 19, and Type A99) in tomato plants acquired from Solanum lycopersicum, Type 99, into sensitive cultivars to nematode infection, such as cultivars (Hybrid F1, 20 rough, and Sam 19) in cucumber plants. Our results prove that some of the plants had been transgenic, while others failed to have the gene transferred to them. In the future experiment, we will proceed to explore more cultivars of nematode-sensitive plants to try to transfer the resistant genes to them using the same technique, chitosan nanoparticles.