Ms. Dona Mwiria

I am a biochemist with a Bachelor of Science, Bachelor of Science Honours, and Master of Science in Biochemistry from the University of Pretoria. My academic background encompasses biochemistry, molecular and cellular biology, microbiology, genetics, protein science, and chemistry.

My research experience includes antimicrobial peptide research, fungal biofilms, and protein–protein interactions. During my Honours research, I investigated whether amino acid substitutions at the interaction interface between InlC from *Listeria monocytogenes* and the SH3-6 domain of human Tuba could strengthen protein–protein binding. My work involved site-directed mutagenesis, recombinant protein expression in *Escherichia coli*, protein purification using affinity and size-exclusion chromatography, and binding analysis using isothermal titration calorimetry. Although the binding measurements did not conclusively establish whether the substitutions strengthened the interaction, the project provided practical experience in experimental protein biochemistry and scientific research documentation.

For my Master's research, I investigated the anti-biofilm activity of tick-derived antimicrobial peptides Os and Os-C against *Candida albicans*. The study examined their ability to inhibit biofilm formation and eradicate established biofilms, alongside investigations into potential mechanisms of antimicrobial activity. My laboratory work included biofilm viability and biomass assays, fluorescence and electron microscopy, membrane-permeability assays, and reactive oxygen species measurements. I presented research findings at scientific meetings, including the Biochemistry Symposium at the University of Pretoria, the South African Society for Biochemistry and Molecular Biology Congress, and the Antimicrobial Peptide Symposium in Montpellier, France, in 2016.

Beyond research, I have several years of experience in university-level teaching and laboratory practical coordination. At the University of Pretoria, I worked as an academic tutor, practical coordinator, and teaching assistant, supporting instruction in biochemistry, molecular and cell biology, and chemistry. My responsibilities included coordinating practical sessions, marking laboratory reports, maintaining academic records, and supporting students and teaching teams. I subsequently worked as a Biology AS and A-Level lecturer and practical coordinator, delivering teaching and organising laboratory practicals, materials, schedules, and resources.

I also engage in science communication and research-based writing, translating scientific concepts into accessible content. My broader professional experience has strengthened my skills in organisation, documentation, coordination, and communication.

My scientific interests include antimicrobial research, molecular and cellular biology, protein interactions, and the investigation of biological mechanisms. I am committed to continued scientific development, expanding my research capabilities, engaging with the wider scientific community, and contributing to scientific knowledge and education.