Degrees:
2016
Doctorate
Other
Publications resulting from Research
Peer-reviewed Publications (in chronological order):
❖ Heba F. Alzan, Audrey O. T. Lau, Donald P. Knowles, David R. Herndon, Massaro W. Ueti, Glen A. Scoles, Lowell S. Kappmeyer, Carlos E. Suarez. Expression of 6-Cys Gene Superfamily Defines Babesia bovis Sexual Stage Development within Rhipicephalus microplus. PLoS One. 2016;11(9).
❖ Heba F. Alzan, Donald P. Knowles, and Carlos E. Suarez. Comparative bioinformatics analysis of transcription factor genes suggests conservation of key regulatory domains among Babesia bovis, B. microti and Theileria equi. PLOS Neglected Tropical Diseases. 2016;10(11).
❖ Heba F. Alzan, Marta Silva, William C. Davis, David R. Herndon, David A. Schneiderand Carlos E. Suarez. Geno- and phenotypic characteristics of a transfected Babesia bovis 6-Cys-E mutant clonal line. Parasites and Vectors. 2017. 10 (1):214.
❖ C.E. Suarez, R.P. Bishop, Heba F. Alzan, W.A. Poole, B.M. Cook. Advances in the application of genetic manipulation methods to Apicomplexan parasites. International Journal for Parasitology. 2017, 47(12):701-710.
❖ Hala E. Hussein, Reginaldo G. Bastos, David A. Schneider, Wendell C. Johnson, Fatma K. Adham, William C. Davis, Jacob M. Laughery, David R. Herndon, Heba F. Alzan, Massaro W. Ueti, Carlos E. Suarez. The Babesia bovis hap2 gene is not required for blood stage replication but expressed upon in vitro sexual stage induction. PLOS Neglected Tropical Diseases. 2017,11(10).
❖ Heba F. Alzan, Cook B.M. and Suarez C.E. Transgenic Babesia bovis lacking 6-Cys sexual-stage genes as the foundation for non-transmissible live vaccines against bovine babesiosis. Tick and tick borne diseases. 2019 ,10 (3):722-728.
❖ Suarez CE, Heba F. Alzan, Silva MG, Rathinasamy V, Poole WA, Cooke BM. Unravelling the cellular and molecular pathogenesis of bovine babesiosis: is the sky the limit. International Journal for Parasitology.2019, 49(2)183-197.
❖ Mona S. Mahmoud, Omnia M. Kandil, Nadia T. Abu El-Ezz, Seham H. M. Hendawy, Bassma S. M. Elsawy, Donald P. Knowles, Reginaldo G. Bastos, Lowell S. Kappmeyer, Jacob M. Laughery, Heba F. Alzan, Carlos E. Suarez. Identification and antigenicity of the Babesia caballi spherical body protein 4 (SBP-4). Parasites and Vectors 2020 13:369.
❖ Sezayi Ozubek, Reginaldo G. Bastos, Heba F. Alzan, Abdullah Inci, Munir Aktas, Carlos E. Suarez. Bovine babesiosis in Turkey: Impact, current gaps and opportunities for intervention. Pathogens 2020, 9, 104.
❖ Monica Florin Christensen, Leonhard Schnittger, Reginaldo G. Bastos, Vignesh A. Rathinasamy, Brian M. Cooke, Heba F. Alzan, and Carlos E. Suarez. Pursuing effective vaccines against livestock diseases. CAB Reviews, 2021, 16, No. 024
❖ Heba F. Alzan, Reginaldo G. Bastos, Massaro W. Ueti, Jacob M. Laughery, Vignesh A. Rathinasamy, Brian M. Cooke, Carlos E. Suarez. Assessment of Babesia bovis 6cys A and 6cys B as components of transmission blocking vaccines for babesiosis. Parasite and Vector, 14, 210 (2021). https://doi.org/10.1186/s13071-021-04712-7.
❖ Bastos, Reginaldo G; Thekkiniath, J; Fuller, Lee; Molestina, Robert G; Florin-Christensen, Monica; Schnittger, Leonhard; Heba F. Alzan; Suarez, Carlos E. Babesia microti Immunoreactive Rhoptry- Associated Protein-1Paralogs Are Ancestral Members of the Piroplasmid-Confined RAP-1 Family. Pathogens, 2021. 10 (11), 1384; https://doi.org/10.3390/pathogens10111384.
❖ Bassma S.M. Elsawy, ahmed nassar, Heba F. Alzan, Raksha V. Bhoora, Sezayi Ozubek, Mona S. Mahmoud, Omnia M. Kandil, Olfat A. Mahdy. Rapid Detection of Equine Piroplasms using multiplex PCR and First genetic characterization of Theileria haneyi in Egypt. Pathogens 2021, 10 (11), 1414; https://doi.org/10.3390/pathogens10111414.
❖ Long Yu, Qin Liu, Wanxin Luo, Heba F. Alzan, Lan He, Junlong Zhao. The Structural Basis of Babesia orientalis Lactate Dehydrogenase (BoLDH). Frontiers in Microbiology- Infectious Agents and Disease, section Infectious Diseases, 2022.
❖ Reginaldo G. Bastos, Heba F. Alzan, Vignesh A. Rathinasamy, Brian M Cooke, Odir A. Delagostin, Raul G. Barletta, Carlos E. Suarez. Harnessing Mycobacterium bovis BCG trained immunity to control human and bovine babesiosis. Vaccines, 2022, 10, 123.
❖ Fatma Gomaa, Zhu-Hong Li, David J, Beaudouin, Heba F. Alzan, Peter R. Girguis, Roberto Docampo, Virginia P. Edgcomb. CRISPR/Cas9-induced disruption of Bodo saltans paraflagellar rod-2 gene reveals its importance for cell survival. Environmental Microbiology- 2022 Jul;24(7):3051-3062.
❖ Heba F. Alzan, Reginaldo G. Bastos, Jacob M Laughery, Glen Scoles, Massaro W Ueti, Wendell C Johnson and Carlos E. Suarez. A Culture-Adapted Strain of Babesia bovis Has Reduced Subpopulation Complexity and Is Unable to Complete Its Natural Life Cycle in Ticks. Frontiers in Microbiology- Infectious Agents and Disease, section Infectious Diseases. 2022. p87.
❖ Sezayi Ö ZÜ BEK, Heba F Alzan, Reginaldo G Bastos, Jacob M Laughery and Carlos E. Suarez. Identification of CCp5 and FNPA as novel non-canonical members of the CCp protein family in Babesia bovis. Frontiers in Veterinary Science, 2022.
❖ Gallenti, R.; Hussein, H.E.; Heba F. Alzan; Suarez, C.E.; Ueti, M.; Asurmendi, S.; Benitez, D.; Araujo, F.R.; Rolls, P.; Sibeko-Matjila, K.; Schnittger, L.; Florin-Christensen, M. U Unraveling the complexity of the rhomboid serine protease 4 family of Babesia bovis using bioinformatics and experimental studies. Pathogens 2022, 11, 344.
❖ Qin Liu, Xueyan Zhan, Dongfang Li, Junlong Zhao, Heba F. Alzan and Lan He. Establishment and application of an indirect enzyme-linked immunosorbent assay for measuring GPI-anchored protein 52 (P52) antibody from Babesia gibsoni infected dog. Animals MDPI 2022, 12 (9),1197.
❖ Reginaldo G Bastos, Jacob M Laughery, Sezayi Ozubek, Heba F Alzan, Naomi S Taus, Massaro W Ueti, Carlos E Suarez. Identification of novel immune correlates of protection against acute bovine babesiosis by superinfecting cattle with in vitro culture attenuated and virulent Babesia bovis strains. Front Immunol. 2022; 13:1045608. The Interaction between Apicomplexan Protozoan and the Host.
❖ Gong H, He L, Alzan HF. Editorial: Exploring new technologies, investigating new targets, and shedding new light on Babesia. Front Cell Infect Microbiol. 2022 Sep 14;12:1004875.
❖ Olfat Mahdy, Ahmed M Nassar, Bassma Elsawy, Heba Alzan, Omnia Mohamed Kandil, Mona S Mahmoud, Carlos Esteban Suarez. Cross-sectional analysis of Piroplasma species infecting camel (Camelus dromedaries) in Egypt using a multipronged molecular diagnostic approach. Frontiers in Veterinary Science 10: 499.
❖ Marta G. Silva, Reginaldo G. Bastos, Jacob M Laughery, Heba F. Alzan, Vignesh A. Rathinasamy, Brian M Cooke, Carlos E. Suarez Vaccination of cattle with the Babesia bovis sexual-stage protein HAP2 abrogates transmission of the parasite by Rhipicephalus microplus ticks. npj Vaccines 8, 140 (2023).
❖ Sezayi Ozubek, Mehmet Can Ulucesme, Reginaldo G. Bastos, Heba F. Alzan, Jacob Laughery, Carlos E. Suarez, Munir Aktas. Experimental infection of healthy and immunosuppressed goats reveals differential pathogenesis of Babesiaaktasi n. sp. Frontiers in Microbiology- Infectious Agents and Disease. Frontiers in cellular and infection microbiology, 12, 827347, 2023.
❖ Bassma S M Elsawy, Mona S Mahmoud, Carlos E Suarez and Heba F. Alzan. Impact of Equine and Camel piroplasmosis in Egypt: how much we know about the current situation? Pathgens. Pathogens 2023, 12, 1318.
❖ Alzan H, Li Y and Gong H (2023) Editorial: Babesia: biology, interactions, and mechanisms of pathogenesis in ticks and its hosts volume II. Frontiers in Cellular and Infection Microbiology 13:1346960.
❖ Seham H.M. Hendawy, Heba F. Alzan, Hoda S. M. Abdel-Ghany, Carlos E Suarez and Gihan Kamel. Novel approach for biochemical comparative analysis of Hyalomma dromedarii salivary gland and mid gut tissues using SR-FTIR. Nature for Scientific report 14, 8515 (2024).
❖ Hoda S.M. Abdel-Ghany, Heba F. Alzan, Seham H.M. Hendawy, Hanan A. Elsadawy, Sobhy Abdel- Shafy. Semi-field application of entomopathogenic nematodes against different developmental stages of the brown dog tick Rhipicephalus sanguineus (Acari: Ixodidae). Acarologia, 2024, 64 (2), pp.323-334.
❖ Bassma S. M. Elsawy, Hoda S. M. Abdel-Ghany, Heba F. Alzan, Sobhy Abdel-Shafy, Yasser Shaein. Molecular detection and genetic characterization of piroplasms and Anaplasmatacae in the camel tick Hyalomma dromedarii collected over different seasons in Egypt. Experimental and Applied Acarology (2024) 10.1007.
❖ Hendawy SHM and Heba F Alzan Editorial: One Health in action: neglected tropical diseases, vector and pest management. Front. Frontiers in Tropical Diseases (2024) 5:1462918.
❖ Rojas, M.J.; Bastos, R.G.; Navas, J.A.; Heba F Alzan; Laughery, J.M.; Lacy, P.A.; Ueti, M.W.; Suarez, C.E. Evaluation of the Use of Sub-Immunodominant Antigens of Babesia bovis with Flagellin C Adjuvant in Subunit Vaccine Development. Vaccines 12, 1215 (2024).
❖ Heba F Alzan, Mona S Mahmoud and Carlos Esteban Suarez. Current vaccines, experimental immunization trials, and new perspectives to control selected vector borne blood parasites of veterinary importance. Frontiers in Veterinary Science(underpublication).
❖ Raihan Rahman, Heba F. Alzan, Jacob M. Laughery, Reginaldo G. Bastos, Massaro W. Ueti1 and Carlos E. Suarez. Structural and antigenic characterization of Babesia bovis Hap2 domains. Nature for Scientific report (submitted).
❖ Heba F. Alzan, Seham HM Hendawy, Bassma SM Elsawy, Hoda SM Abdel-Ghan, Sobhy Abdel-Shafy, Tetsuya Tanaka, Mona S. Mahmoud. Immunological characterization of aquaporin 1 peptide and ferritin 2 recombinant protein for vaccine development against Rhipicephalus sanguineus tick. (Under prepration).
❖ Heba F. Alzan and Carlos E. Suarez. Defining CLAMP, a pan-apicomplexan microneme protein,as a novel vaccine candidate against Babesia bovis. Under preparation.
❖ Heba F. Alzan, Audrey O. T. Lau, Donald P. Knowles, David R. Herndon, Massaro W. Ueti, Glen A. Scoles, Lowell S. Kappmeyer, Carlos E. Suarez. Expression of 6-Cys Gene Superfamily Defines Babesia bovis Sexual Stage Development within Rhipicephalus microplus. PLoS One. 2016;11(9).
❖ Heba F. Alzan, Donald P. Knowles, and Carlos E. Suarez. Comparative bioinformatics analysis of transcription factor genes suggests conservation of key regulatory domains among Babesia bovis, B. microti and Theileria equi. PLOS Neglected Tropical Diseases. 2016;10(11).
❖ Heba F. Alzan, Marta Silva, William C. Davis, David R. Herndon, David A. Schneiderand Carlos E. Suarez. Geno- and phenotypic characteristics of a transfected Babesia bovis 6-Cys-E mutant clonal line. Parasites and Vectors. 2017. 10 (1):214.
❖ C.E. Suarez, R.P. Bishop, Heba F. Alzan, W.A. Poole, B.M. Cook. Advances in the application of genetic manipulation methods to Apicomplexan parasites. International Journal for Parasitology. 2017, 47(12):701-710.
❖ Hala E. Hussein, Reginaldo G. Bastos, David A. Schneider, Wendell C. Johnson, Fatma K. Adham, William C. Davis, Jacob M. Laughery, David R. Herndon, Heba F. Alzan, Massaro W. Ueti, Carlos E. Suarez. The Babesia bovis hap2 gene is not required for blood stage replication but expressed upon in vitro sexual stage induction. PLOS Neglected Tropical Diseases. 2017,11(10).
❖ Heba F. Alzan, Cook B.M. and Suarez C.E. Transgenic Babesia bovis lacking 6-Cys sexual-stage genes as the foundation for non-transmissible live vaccines against bovine babesiosis. Tick and tick borne diseases. 2019 ,10 (3):722-728.
❖ Suarez CE, Heba F. Alzan, Silva MG, Rathinasamy V, Poole WA, Cooke BM. Unravelling the cellular and molecular pathogenesis of bovine babesiosis: is the sky the limit. International Journal for Parasitology.2019, 49(2)183-197.
❖ Mona S. Mahmoud, Omnia M. Kandil, Nadia T. Abu El-Ezz, Seham H. M. Hendawy, Bassma S. M. Elsawy, Donald P. Knowles, Reginaldo G. Bastos, Lowell S. Kappmeyer, Jacob M. Laughery, Heba F. Alzan, Carlos E. Suarez. Identification and antigenicity of the Babesia caballi spherical body protein 4 (SBP-4). Parasites and Vectors 2020 13:369.
❖ Sezayi Ozubek, Reginaldo G. Bastos, Heba F. Alzan, Abdullah Inci, Munir Aktas, Carlos E. Suarez. Bovine babesiosis in Turkey: Impact, current gaps and opportunities for intervention. Pathogens 2020, 9, 104.
❖ Monica Florin Christensen, Leonhard Schnittger, Reginaldo G. Bastos, Vignesh A. Rathinasamy, Brian M. Cooke, Heba F. Alzan, and Carlos E. Suarez. Pursuing effective vaccines against livestock diseases. CAB Reviews, 2021, 16, No. 024
❖ Heba F. Alzan, Reginaldo G. Bastos, Massaro W. Ueti, Jacob M. Laughery, Vignesh A. Rathinasamy, Brian M. Cooke, Carlos E. Suarez. Assessment of Babesia bovis 6cys A and 6cys B as components of transmission blocking vaccines for babesiosis. Parasite and Vector, 14, 210 (2021). https://doi.org/10.1186/s13071-021-04712-7.
❖ Bastos, Reginaldo G; Thekkiniath, J; Fuller, Lee; Molestina, Robert G; Florin-Christensen, Monica; Schnittger, Leonhard; Heba F. Alzan; Suarez, Carlos E. Babesia microti Immunoreactive Rhoptry- Associated Protein-1Paralogs Are Ancestral Members of the Piroplasmid-Confined RAP-1 Family. Pathogens, 2021. 10 (11), 1384; https://doi.org/10.3390/pathogens10111384.
❖ Bassma S.M. Elsawy, ahmed nassar, Heba F. Alzan, Raksha V. Bhoora, Sezayi Ozubek, Mona S. Mahmoud, Omnia M. Kandil, Olfat A. Mahdy. Rapid Detection of Equine Piroplasms using multiplex PCR and First genetic characterization of Theileria haneyi in Egypt. Pathogens 2021, 10 (11), 1414; https://doi.org/10.3390/pathogens10111414.
❖ Long Yu, Qin Liu, Wanxin Luo, Heba F. Alzan, Lan He, Junlong Zhao. The Structural Basis of Babesia orientalis Lactate Dehydrogenase (BoLDH). Frontiers in Microbiology- Infectious Agents and Disease, section Infectious Diseases, 2022.
❖ Reginaldo G. Bastos, Heba F. Alzan, Vignesh A. Rathinasamy, Brian M Cooke, Odir A. Delagostin, Raul G. Barletta, Carlos E. Suarez. Harnessing Mycobacterium bovis BCG trained immunity to control human and bovine babesiosis. Vaccines, 2022, 10, 123.
❖ Fatma Gomaa, Zhu-Hong Li, David J, Beaudouin, Heba F. Alzan, Peter R. Girguis, Roberto Docampo, Virginia P. Edgcomb. CRISPR/Cas9-induced disruption of Bodo saltans paraflagellar rod-2 gene reveals its importance for cell survival. Environmental Microbiology- 2022 Jul;24(7):3051-3062.
❖ Heba F. Alzan, Reginaldo G. Bastos, Jacob M Laughery, Glen Scoles, Massaro W Ueti, Wendell C Johnson and Carlos E. Suarez. A Culture-Adapted Strain of Babesia bovis Has Reduced Subpopulation Complexity and Is Unable to Complete Its Natural Life Cycle in Ticks. Frontiers in Microbiology- Infectious Agents and Disease, section Infectious Diseases. 2022. p87.
❖ Sezayi Ö ZÜ BEK, Heba F Alzan, Reginaldo G Bastos, Jacob M Laughery and Carlos E. Suarez. Identification of CCp5 and FNPA as novel non-canonical members of the CCp protein family in Babesia bovis. Frontiers in Veterinary Science, 2022.
❖ Gallenti, R.; Hussein, H.E.; Heba F. Alzan; Suarez, C.E.; Ueti, M.; Asurmendi, S.; Benitez, D.; Araujo, F.R.; Rolls, P.; Sibeko-Matjila, K.; Schnittger, L.; Florin-Christensen, M. U Unraveling the complexity of the rhomboid serine protease 4 family of Babesia bovis using bioinformatics and experimental studies. Pathogens 2022, 11, 344.
❖ Qin Liu, Xueyan Zhan, Dongfang Li, Junlong Zhao, Heba F. Alzan and Lan He. Establishment and application of an indirect enzyme-linked immunosorbent assay for measuring GPI-anchored protein 52 (P52) antibody from Babesia gibsoni infected dog. Animals MDPI 2022, 12 (9),1197.
❖ Reginaldo G Bastos, Jacob M Laughery, Sezayi Ozubek, Heba F Alzan, Naomi S Taus, Massaro W Ueti, Carlos E Suarez. Identification of novel immune correlates of protection against acute bovine babesiosis by superinfecting cattle with in vitro culture attenuated and virulent Babesia bovis strains. Front Immunol. 2022; 13:1045608. The Interaction between Apicomplexan Protozoan and the Host.
❖ Gong H, He L, Alzan HF. Editorial: Exploring new technologies, investigating new targets, and shedding new light on Babesia. Front Cell Infect Microbiol. 2022 Sep 14;12:1004875.
❖ Olfat Mahdy, Ahmed M Nassar, Bassma Elsawy, Heba Alzan, Omnia Mohamed Kandil, Mona S Mahmoud, Carlos Esteban Suarez. Cross-sectional analysis of Piroplasma species infecting camel (Camelus dromedaries) in Egypt using a multipronged molecular diagnostic approach. Frontiers in Veterinary Science 10: 499.
❖ Marta G. Silva, Reginaldo G. Bastos, Jacob M Laughery, Heba F. Alzan, Vignesh A. Rathinasamy, Brian M Cooke, Carlos E. Suarez Vaccination of cattle with the Babesia bovis sexual-stage protein HAP2 abrogates transmission of the parasite by Rhipicephalus microplus ticks. npj Vaccines 8, 140 (2023).
❖ Sezayi Ozubek, Mehmet Can Ulucesme, Reginaldo G. Bastos, Heba F. Alzan, Jacob Laughery, Carlos E. Suarez, Munir Aktas. Experimental infection of healthy and immunosuppressed goats reveals differential pathogenesis of Babesiaaktasi n. sp. Frontiers in Microbiology- Infectious Agents and Disease. Frontiers in cellular and infection microbiology, 12, 827347, 2023.
❖ Bassma S M Elsawy, Mona S Mahmoud, Carlos E Suarez and Heba F. Alzan. Impact of Equine and Camel piroplasmosis in Egypt: how much we know about the current situation? Pathgens. Pathogens 2023, 12, 1318.
❖ Alzan H, Li Y and Gong H (2023) Editorial: Babesia: biology, interactions, and mechanisms of pathogenesis in ticks and its hosts volume II. Frontiers in Cellular and Infection Microbiology 13:1346960.
❖ Seham H.M. Hendawy, Heba F. Alzan, Hoda S. M. Abdel-Ghany, Carlos E Suarez and Gihan Kamel. Novel approach for biochemical comparative analysis of Hyalomma dromedarii salivary gland and mid gut tissues using SR-FTIR. Nature for Scientific report 14, 8515 (2024).
❖ Hoda S.M. Abdel-Ghany, Heba F. Alzan, Seham H.M. Hendawy, Hanan A. Elsadawy, Sobhy Abdel- Shafy. Semi-field application of entomopathogenic nematodes against different developmental stages of the brown dog tick Rhipicephalus sanguineus (Acari: Ixodidae). Acarologia, 2024, 64 (2), pp.323-334.
❖ Bassma S. M. Elsawy, Hoda S. M. Abdel-Ghany, Heba F. Alzan, Sobhy Abdel-Shafy, Yasser Shaein. Molecular detection and genetic characterization of piroplasms and Anaplasmatacae in the camel tick Hyalomma dromedarii collected over different seasons in Egypt. Experimental and Applied Acarology (2024) 10.1007.
❖ Hendawy SHM and Heba F Alzan Editorial: One Health in action: neglected tropical diseases, vector and pest management. Front. Frontiers in Tropical Diseases (2024) 5:1462918.
❖ Rojas, M.J.; Bastos, R.G.; Navas, J.A.; Heba F Alzan; Laughery, J.M.; Lacy, P.A.; Ueti, M.W.; Suarez, C.E. Evaluation of the Use of Sub-Immunodominant Antigens of Babesia bovis with Flagellin C Adjuvant in Subunit Vaccine Development. Vaccines 12, 1215 (2024).
❖ Heba F Alzan, Mona S Mahmoud and Carlos Esteban Suarez. Current vaccines, experimental immunization trials, and new perspectives to control selected vector borne blood parasites of veterinary importance. Frontiers in Veterinary Science(underpublication).
❖ Raihan Rahman, Heba F. Alzan, Jacob M. Laughery, Reginaldo G. Bastos, Massaro W. Ueti1 and Carlos E. Suarez. Structural and antigenic characterization of Babesia bovis Hap2 domains. Nature for Scientific report (submitted).
❖ Heba F. Alzan, Seham HM Hendawy, Bassma SM Elsawy, Hoda SM Abdel-Ghan, Sobhy Abdel-Shafy, Tetsuya Tanaka, Mona S. Mahmoud. Immunological characterization of aquaporin 1 peptide and ferritin 2 recombinant protein for vaccine development against Rhipicephalus sanguineus tick. (Under prepration).
❖ Heba F. Alzan and Carlos E. Suarez. Defining CLAMP, a pan-apicomplexan microneme protein,as a novel vaccine candidate against Babesia bovis. Under preparation.