Sayma Serine Chompa

Abstract of the Annual Plant Tissue Culture & Biotechnology Conference
BSRI, Ishurdi, Pabna, 6-7 April, 2018
Virus Free Potato Mini-Tuber Production through Meristem
Culture
Sayma Serine Chopma1 and Md. Belal Hossain2
Plant Tissue Culture Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207, Bangladesh

Experiments were conducted with a view to establish a protocol for virus free mini-tubers production, in vitro, using potato meristem as explant collected from potato sprouts of three popular potato varieties Diamont, Cardinal, Granola. In the present study four levels of GA3 (100, 200, 300 and 400 ppm) was used to assess the influence on sprouting abilities in three selected potato verities. The maximum sprouting efficiency was observed in 400ppm GA3 treatment within short period of time in Granola variety. The effect of different combination and concentration of IBA and BAP was used along with fresh MS media to inoculate meristems of potato sprouts. IBA concentration were 1mg/L, 1.5mg/Land BAP concentration were 0.5 mg/L, 1.0 mg/L, 1.5 mg/L and 2mg/L. The effect of combined hormones was also studied. The Diamond variety showed the maximum callus size (1.98cm) within a very short period of time (7 days) while treated with IBA at the concentration of 1.0 mg/L and BAP at 1.5 mg/L. Granola meristem inoculated in hormonal treatment showed the best results regarding minimum days required to shoot initiation (6 days) followed by Cardinal (8 days) and Diamond (9 days). The highest number of shoots/plantlet (4 shoots/plantlet) and the longest plantlet (6.3cm) was found in Granola variety followed by Cardinal (3.0 shoots/plantlet and 5.8cm) and Diamond (3 shoots/plantlet and 4.5cm). Maximum numbers of leaves were also found in Granola variety (16 leaves) in the treatment combination of 1.0 mg/L IBA with 1.5 mg/L BAP. Granola gave maximum performance in respect of maximum number of root (15 roots/plantlets) within a short time of root initiation (20 days), followed by Cardinal (7.0 roots/plantlets) of root initiation (21 days) and Diamond (6 roots/plantlets) of root initiation (23 days). Granola produced the longest (6.59 cm) roots/plantlet. Rooted plantlets were gradually acclimatized and successfully established in the field. However, in overall observations in this study, Granola showed better performance from meristem tissue culture and all plants were found normal and free from potato viruses that were tested through ELISA test.